Part:BBa_K4451008
T4 Alc
BBa_K4451008 encodes the Alc protein from T4 phage. This protein has been reported to interact with the RNA polymerase beta prime subunit and cause host transcription shutoff (Kashlev et al., 1993).
Description
iGEM Sheffield 2023- PARSE
A transcriptional terminator from T4 bacteriophage that has been shown to suppress growth rate in E. coli. The Alc gene encodes a site-specific termination factor, which binds to commonly found sites in the E. coli genome and causes early transcriptional termination, thus reducing transcriptional output and reducing growth rate. Termination is only possible on actively processing RNA polymerases, any stall within 15bp of the Alc site abolishes early termination. This, alongside AsiA (BBa_K4451010), is utilised to trigger transcriptional redirection toward phage genes in the T4 infection cycle (Kashlev et al., 1993).
Figure 1: Mean log2(OD) against time for Alc
From Figure 1, ALc shows a slightly lower mean log2OD compare to vsfGFP after around 7.5 hours. The different [IPTG]/different levels of induction of Alc doesn't show any significant difference in the mean log2OD. This data is inconclusive as to whether Alc protein expression slows the growth of E. coli in comparison to the expression of vsfGFP.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 7
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 7
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 413
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 7
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 7
- 1000COMPATIBLE WITH RFC[1000]
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